Biomolecular Dynamics in Cell Signaling

Dynamics of multiprotein complexes in cellular signaling

Our research focuses on describing the effects of reversible, covalent phosphorylation (His-Asp two component signalling) and noncovalent phosphates (cyclic nucleotide monophosphate (cAMP), ATP, ADP and AMP) on protein conformational dynamics. Ongoing research areas include role of phosphodiesterases (PDEs) in cAMP homeostasis and allosteric effects of histidine phosphorylation and phosphotransfer to aspartates in two-component signalling.

Dynamics of transmembrane signal transduction

We seek to probe the dynamics of membrane proteins with an emphasis on signaling receptors with transmembrane domains by structural mass spectrometry. This project focuses EnvZ osmosensing receptor, describing lipid-mediated changes in receptor dynamics and transmembrane signaling.